Molecular Mechanisms of Induced Pluripotency

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Abstract

In this review the distinct aspects of somatic cell reprogramming are discussed. The molecular mechanisms of generation of induced pluripotent stem (iPS) cells from somatic cells via the introduction of transcription factors into adult somatic cells are considered. Particular attention is focused on the generation of iPS cells without genome modifications via the introduction of the mRNA of transcription factors or the use of small molecules. Furthermore, the strategy of direct reprogramming of somatic cells omitting the generation of iPS cells is considered. The data concerning the differences between ES and iPS cells and the problem of epigenetic memory are also discussed. In conclusion, the possibility of using iPS cells in regenerative medicine is considered.

About the authors

I. A. Muchkaeva

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Author for correspondence.
Email: izomerizaciya@list.ru
Russian Federation

E. B. Dashinimaev

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: izomerizaciya@list.ru
Russian Federation

V. V. Terskikh

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: izomerizaciya@list.ru
Russian Federation

Yu. V. Sukhanov

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: izomerizaciya@list.ru
Russian Federation

A. V. Vasiliev

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: izomerizaciya@list.ru
Russian Federation

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