Reconstruction of Rabbit Urethral Epithelium with Skin Keratinocytes

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We have investigated the living skin equivalent (LSE) as an alternative source of plastic material for closing full-thickness epithelial-stromal urethral injuries. The possibility of transdifferentiation of epidermal keratinocytes, a component of 3D tissue constructs, was investigated in vivo in a model of the recovery of urethral injuries in laboratory rabbits. Autologous grafting of LSE in de-epithelialized urethra showed that skin keratinocytes placed in a specific in vivo microenvironment can be incorporated into the damaged area and function as urothelium. The use of EGFP transfected keratinocytes allowed us to identify transplanted cells. The reconstructed urethral tubes did not develop strictures or fistulas at the site of the grafted LSE. Immunohistochemical studies of neo-urothelium revealed EGFP-positive cells expressing the urothelial markers K7 and UP3.

About the authors

O. S. Rogovaya

N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences; N.I. Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation

Author for correspondence.
Russian Federation

A. K. Fayzulin

Morozov Children’s Clinical Hospital, Moscow Department of Health Care

Russian Federation

A. V. Vasiliev

N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences

Russian Federation

A. V. Kononov

A.I. Evdokimov State University of Medicine and Dentistry, Ministry of Healthcare of the Russian Federation,

Russian Federation

V. V. Terskikh

N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences

Russian Federation


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Copyright (c) 2015 Rogovaya O.S., Fayzulin A.K., Vasiliev A.V., Kononov A.V., Terskikh V.V.

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