Influence of Ion Strength and pH on Thermal Stability of Yeast Formate Dehydrogenase

Cover Page

Abstract


The kinetics of the thermal inactivation of recombinant wild-type formate dehydrogenase from Candida boidinii yeast was studied in the temperature range of 53-61 oC and pH 6.0, 7.0, and 8.0. It was shown that the loss of the enzyme’s activity proceeds via a monomolecular mechanism. Activation parameters ∆≠ and ∆S≠ were calculated based on the temperature relations dependence of inactivation rate constants according to the transition state theory. Both parameters are in a range that corresponds to globular protein denaturation processes. Optimal conditions for the stability of the enzyme were high concentrations of the phosphate buffer or of the enzyme substrate sodium formate at pH = 7.0.

Influence of Ion Strength and pH on Thermal Stability of Yeast Formate Dehydrogenase

V I Tishkov

Lomonosov Moscow State University; Innovations and High Technologies MSU Ltd; Bach Institute of Biochemistry, Russian Academy of Sciences

Email: vitishkov@gmail.com
Department of Chemical Enzymology, Faculty of Chemistry

S V Uglanova

Lomonosov Moscow State University; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Department of Chemical Enzymology, Faculty of Chemistry

V V Fedorchuk

Lomonosov Moscow State University

Department of Chemical Enzymology, Faculty of Chemistry

S S Savin

Innovations and High Technologies MSU Ltd; Bach Institute of Biochemistry, Russian Academy of Sciences

  1. Vinals C., Depiereux E., Feytmans E. // Biochem. Biophys. Res. Commun. 1993. V. 192. P. 182-188.
  2. Tishkov V.I., Popov V.O. // Biomol. Eng. 2006. V. 23. P. 89-110.
  3. Lamzin V.S., Dauter Z., Popov V.O., et al. // J. Mol. Biol. 1994. V. 236. P. 759-785.
  4. Filippova E.V., Polyakov K.M., Tikhonova T.V., et al. // Kristallographia. 2006. V. 51. P. 627-631.
  5. Schirwitz K., Schmidt A., Lamzin V.S. // Protein Sci. 2007. V. 16. P. 1146-1156.
  6. Sadykhov E.G., Serov A.E., Yasnyi I.E, et al. // Vestnik MGU. 2 Ed. Khimia. 2006. V. 47. P. 31-34.
  7. Shabalin I.G., Serov A.E., Skirello O.E. et al. // Crystallography Reports . 2010. V.55. N5. P.855-859
  8. Fedorchuk V.V., Galkin A.G., Yasny I.E. et al. // Biochemistry (Moscow). 2002. V. 67. P. 1145-1151.
  9. Bommarius A.S., Schwarm M., Stingl K., et al. // Tetrahedron-Asymmetry. 1995. V. 6. P. 2851-2888.
  10. Rojkova A.M., Galkin A.G., Kulakova L.B., et al. // FEBS Lett. 1999. V. 445. P. 183-188.
  11. Felber S. Optimierung der NAD-abhangigen Formiatdehydrogenase aus Candida boidinii fur den Einsatz in der Biokatalyse: PhD Thesis. Dusseldorf: Heinrich-Heine University of Dusseldorf, 2001. URL: http://diss.ub.uni-duesseldorf.de/ebib/diss/file?dissid=78
  12. Sadykhov E., Serov A., Voinova N., et al. // Appl. Biochem. Microbiol. 2006. V. 42. P. 236-240.

Views

Abstract - 431

PDF (English) - 156

PDF (Russian) - 188

Cited-By


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

PlumX

Dimensions

Refbacks

  • There are currently no refbacks.

Copyright (c) 2010 Tishkov V.I., Uglanova S.V., Fedorchuk V.V., Savin S.S.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies