An Efficient Method for the Delivery of the Interleukin-2 Gene to Human Hematopoietic Cells using the Fiber-Modified Recombinant Adenovirus

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  • Authors: Rogozhin VN1,2, Logunov DY.1, Shchebliakov DV1, Shmarov MM1, Khodunova EE3, Galtseva IV3, Belousova RV2, Naroditsky BS1, Gintsburg AL1
  • Affiliations:
    1. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation
    2. Moscow state academy of veterinary medicine and biotechnology named K.I. Skryabin
    3. National Research Center for Hematology, Ministry of Health and Social Development of the Russian Federation
  • Issue: Vol 3, No 3 (2011)
  • Pages: 100-106
  • Section: Articles
  • URL: http://actanaturae.ru/2075-8251/article/view/10705
  • DOI: https://doi.org/10.32607/20758251-2011-3-3-100-106
  • Cite item

Abstract


Recombinant human adenovirus serotype 5 (Ad5/35F-IL2) with modified fibres containing the C-terminal domain fiber-knob of human adenovirus serotype 35, carrying the gene of recombinant human IL-2, has been designed. As a result of the fiber modification, the adenovirus can efficiently deliver the genetic information to bone marrow leukocytes and the tumor blood cells KG-1A (human myeloblastic leukemia cells) and U937 (human histiocytic lymphoma cells), which are normally resistant to Ad5 infection. The flow cytometry data reveal that the modified Ad5/35F penetrates into a population of monocytes, granulocytes, and blast cells of human bone marrow. The expression of interleukin-2 in CAR-negative bone marrow leukocytes (3682.52 ± 134.21 pg/ml) and the cell lines KG-1A (748.3 ± 32.8 pg/ml) and U937 (421.5 ± 59.4 pg/ ml) transduced with adenovirus Ad5/35F-IL2 is demonstrated. The fiber-modified adenovirus can be used as a vector for the efficient gene delivery of interleukin-2 to human normal and tumor hematopoietic cells. KEYWORDS adenovial vector; pseudotyping; interleukin-2; CD46; capsid modification.

An Efficient Method for the Delivery of the Interleukin-2 Gene to Human Hematopoietic Cells using the Fiber-Modified Recombinant Adenovirus

V N Rogozhin

Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation; Moscow state academy of veterinary medicine and biotechnology named K.I. Skryabin

Email: Rogojin_V@mail.ru

D Yu Logunov

Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation

D V Shchebliakov

Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation

M M Shmarov

Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation

E E Khodunova

National Research Center for Hematology, Ministry of Health and Social Development of the Russian Federation

I V Galtseva

National Research Center for Hematology, Ministry of Health and Social Development of the Russian Federation

R V Belousova

Moscow state academy of veterinary medicine and biotechnology named K.I. Skryabin

B S Naroditsky

Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation

A L Gintsburg

Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of the Russian Federation

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Copyright (c) 2011 Rogozhin V.N., Logunov D.Y., Shchebliakov D.V., Shmarov M.M., Khodunova E.E., Galtseva I.V., Belousova R.V., Naroditsky B.S., Gintsburg A.L.

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