Effective Genetic Expression of Nanoantibodies by Recombinant Adenoviral Vector in vitro

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Abstract


The present study is devoted to the feasibility of expressing the single-domain mini-antibody (nanoantibody) selected from the library of sequences of the variable domains of special single-stranded antibodies derived from an immunized camel, a gene of which was introduced into eukaryotic cells within a recombinant adenoviral vector. A vector bearing the gene of a single-domain nanoantibody was obtained using the AdEasy Adenoviral Vector System (Stratagene). This method of delivering the nanoantibody gene facilitates efficient expression of this gene and functional activity of the nanoantibody. The results obtained can be used to produce passive immunizing tools against pathogens or new-generation immunobiological antitoxic medication.

Effective Genetic Expression of Nanoantibodies by Recombinant Adenoviral Vector in vitro

I Yu Gribova

Gamaleya Research Institute for Epidemiology and Microbiology

S V Tillib

Institute of Gene Biology, Russian Academy of Sciences

I L Tutykhina

Gamaleya Research Institute for Epidemiology and Microbiology

М М Shmarov

Gamaleya Research Institute for Epidemiology and Microbiology

D Yu Logunov

Gamaleya Research Institute for Epidemiology and Microbiology

L V Verkhovskaya

Gamaleya Research Institute for Epidemiology and Microbiology

B S Naroditskii

Gamaleya Research Institute for Epidemiology and Microbiology

Email: bsnar1941@yahoo.com

A L Gintsburg

Gamaleya Research Institute for Epidemiology and Microbiology

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Copyright (c) 2011 Gribova I.Y., Tillib S.V., Tutykhina I.L., Shmarov М.М., Logunov D.Y., Verkhovskaya L.V., Naroditskii B.S., Gintsburg A.L.

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