Screening of Potential HIV-1 Inhibitors/ Replication Blockers Using Secure Lentiviral in Vitro System

Cover Page

Cite item

Full Text

Abstract

The development and usage of safe cell systems for testing agents which possess anti-HIV activity is a very important factor in the design of new drugs. We have described in detail a system we designed that is based on lentiviral vectors (Prokofjeva et. al., Antiviral Therapy, in print) for swift and completely safe screening of potential HIV-1 replication inhibitors. The system enables one to test the efficiency of the inhibitory activity of compounds whose action is directed towards either wild-type HIV-1 reverse transcriptase or integrase, or mutant enzymes corresponding to the drug-resistant virus form. Testing results of a number of already known drugs, which correlate well with published data as well as data on newly synthesized compounds, were obtained. Application of this system substantially broadens the possibilities of preclinical anti-HIV drugs testing

Full Text

Screening of Potential HIV-1 Inhibitors/ Replication Blockers Using Secure Lentiviral in Vitro System
×

About the authors

M M Prokofjeva

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

P V Spirin

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

D V Yanvarev

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

A V Ivanov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

M S Novikov

Volgograd State Medical University

O A Stepanov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

M B Gottikh

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

S N Kochetkov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

B Fehse

Research Department Cell and Gene Therapy

Department for Stem Cell Transplantation University Medical Center Hamburg-Eppendorf

C Stocking

Heinrich-Pette-Institute for Experimental Virology and Immunology

V S Prassolov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: prassolov45@mail.ru

References

  1. Report on the global AIDS epidemic, World Health Organization.
  2. http://www.hivrussia.org/stat/2009/10.shtml
  3. De Clercq E. // Int. J. Antimicrob. Agents. 2009. V. 33. № 4. P. 307-320.
  4. Kuritzkes D.R., Walker B.D. // Fields Virology / Eds Knipe D.M., Howley P.M. Philadelphia: Lippinkot, Williams & Wilkins, 2007.
  5. Hengge U.R., Ruzicka T., Tyring S.K., Stuschke M., Roggendorf M., Schwartz R.A., Seeber S. // Lancet Infect. Dis. 2002. V. 2. № 5. P. 281-292.
  6. Wittkop L., Günthard H.F., de Wolf F., Dunn D., Cozzi-Lepri A., de Luca A., Kücherer C., Obel N., von Wyl V., Masquelier B., et al. // Lancet Infect. Dis. 2011. V. 11. № 5. P. 363-371.
  7. Núñez M. // Hepatology. 2010. V. 52. № 3. P. 1143-1155.
  8. Izzedine H., Harris M., Perazella M.A. // Nat. Rev. Nephrol. 2009. V. 5. № 10. P. 563-573.
  9. Panyutich A.V., Prassolov V.S., Shydlovskaya E.A., Reznikov M.V., Chumakov P.M., Voitenuk N.N. // Hybridoma. 1990. V. 9. № 4. P. 401-406.
  10. Prassolov V.S., Meyer J., Brandenburg G., Hannemann J., Bergemann J., Ostertag W., Stocking C. // Exp. Hematol. 2001. V. 29. № 6. P. 756-765.
  11. Spirin P.V., Vilgelm A.E., Prassolov V.S. // Mol. Biol. (Mosk). 2008. V. 42(5). P. 814-825.
  12. Spirin P.V., Baskaran D., Rubtsov P.M., Zenkova M.A., Vlassov V.V., Chernolovskaya E.L., Prassolov V.S. // Acta Naturae. 2009. V. 2. № 2 (2). P. 86-90.
  13. Spirin P.V., Baskaran D., Orlova N.N., Rulina A.V., Nikitenko N.A., Chernolovskaya E.L., Zenkova M.A., Vlassov V.V., Rubtsov P.M., Chumakov P.M., et al // Mol. Biol. (Mosk). 2010. V. 44(5). P. 776-786.
  14. Kellam P., Larder B. //Antimicrob. Agents Chemother. 1994. V. 38. № 1. P. 23-30.
  15. Walter H., Schmidt B., Korn K., Vandamme A.M., Harrer T., Uberla K. // J. Clin. Virol. 1999. V. 13. № 1-2. P. 71-80.
  16. Jarmy G., Heinkelein M., Weissbrich B., Jassoy C., Rethwilm A. // J. Med. Virol. 2001. V. 64. № 3. P. 223-231.
  17. Garcia-Perez J., Sanchez-Palomino S., Perez-Olmeda M., Fernandez B., Alcami J. // J. Med. Virol. 2007. V. 79. № 2. P. 127-137.
  18. Cheresiz S.V., Grigoryev I.V., Semenova E.A., Pustylnyak V.O., Vlasov V.V., Pokrovsky A.G. // Dokl. Biochem. Biophys. 2010. V. 435. P. 295-298.
  19. Prassolov V.S., Chumakov P.M. Vector pPS-1-neo for gene transfer and expression in cultivated somatic mammalian cells. Inventor’s certificate № 1440036 from 26.06.1987. Application 4268094. (Russian).
  20. Rosenblum L.L., Patton G., Grigg A.R., Frater A. J., Cain D., Erlwein O., Hill C.L., Clarke J.R., McClure M.O. // Antivir. Chem. Chemother. 2001. V. 12. № 2. P. 91-97.
  21. Smith R.A., Gottlieb G.S., Miller A.D. // Retrovirology. 2010. V. 7. P. 70-81.
  22. Bjerke M., Franco M., Johansson M., Balzarini J., Karlsson A. // Biochem. Pharmacol. 2008. V. 75. № 6. P. 1313-1321.
  23. Perez-Bercoff D., Wurtzer S., Compain S., Benech H., Clavel F. // J. Virol. 2007. V. 81. № 9. P. 4540-4550.
  24. Patick A.K., Boritzki T. J., Bloom L.A. // Antimicrob. Agents Chemother. 1997. V. 41. № 10. P. 2159-2164.
  25. Grob P.M., Wu J.C., Cohen K.A., Ingraham R.H., Shih C.K., Hargrave K.D., McTague T.L., Merluzzi V.J. // AIDS Res. Hum. Retroviruses. 1992. V. 8. № 2. P. 145-152.
  26. Witvrouw M., Arranz M.E., Pannecouque C., Declercq R., Jonckheere H., Schmit J.C., Vandamme A.M., Diaz J.A., Ingate S.T., Desmyter J., et al. // Antimicrob. Agents Chemother. 1998. V. 42. № 3. P. 618-623.
  27. Novikov M.S., Ivanova O.N., Ivanov A.V., Ozerov A.A., Valuev-Elliston V.T., Gurskaya G.V., Kochetkov S.N., Pannecouque C., Balzarini J., Seley-Radtke K.L. // Bioorg. Med. Chem. 2011. V. 19. № 19. P. 5794-5802.
  28. Hazuda D.J., Felock P., Witmer M., Wolfe A., Stillmock K., Grobler J.A., Espeseth A., Gabryelski L., Schleif W., Blau C., et al. // Science. 2000. V. 287. № 5453. P. 646-650.
  29. Paprotka T., Venkatachari N.J., Chaipan C., Burdick R., Delviks-Frankenberry K.A., Hu W.S., Pathak V.K. // J. Virol. 2010. V. 84. № 11. P. 5719-5729.
  30. Gröschel B., Höver G., Doerr H.W., Cinatl J., Jr. // Nucleosides Nucleotides Nucleic Acids. 2001. V. 20. № 4-7. P. 487-492.
  31. Paintsil E., Dutschman G.E., Hu R., Grill S.P., Wang C.J., Lam W., Li F.Y., Ghebremichael M., Northrup V., Cheng Y.C. // Antimicrob. Agents Chemother. 2011. V. 55. № 2. P. 895-903.
  32. Summa V., Petrocchi A., Bonelli F., Crescenzi B., Donghi M., Ferrara M., Fiore F., Gardelli C., Gonzalez Paz O., Hazuda D.J., et al. // J. Med. Chem. 2008. V. 51. № 18. P. 5843-5855.
  33. Arion D., Parniak M.A. // Drug Resist. Updat. 1999. V. 2. № 2. P. 91-95.
  34. Isaguliants M.G., Belikov S.V., Starodubova E.S., Gizatullin R.Z., Rollman E., Zuber B., Zuber A.K., Grishchenko O.I., Rytting A.S., Kallander C.F., et al. // AIDS Res. Hum. Retroviruses. 2004. V. 20. № 2. P. 191-201.
  35. Cruchaga C., Ansó E., Rouzaut A., Martínez-Irujo J.J. // J. Biol. Chem. 2006. V. 281. № 38. P. 27744-27752.
  36. Song Y., Chan J.M.W., Tovian Z., Secrest A., Nagy E., Krysiak K., Bergan K., Parniak M.A., Oldfield E. // Bioorg. Med. Chem. 2008. V. 16. P. 8959-8967.
  37. Jacobson M.A., van der Horst C., Causey D.M., Dehlinger M., Hafner R., Mills J. // J. Infect Dis. 1991. V. 163. № 6. P. 1219-1222.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2011 Prokofjeva M.M., Spirin P.V., Yanvarev D.V., Ivanov A.V., Novikov M.S., Stepanov O.A., Gottikh M.B., Kochetkov S.N., Fehse B., Stocking C., Prassolov V.S.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies