Cytotoxic and Immunochemical Properties of Viscumin Encapsulated in Polylactide Microparticles

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Abstract


Biodegradable polylactide microparticles with encapsulated cytotoxic protein viscumin were obtained via the ultrasound-assisted supercritical fluid technique. The size of the microparticles was 10-50 μМ, as shown by electron microscopy. The time course of viscumin release from microparticles was studied using an immunoenzyme test system with anti-viscumin monoclonal antibodies. It was found that 99.91% of the cytotoxic protein was incorporated into polymer microparticles. Only 0.08% of the initially encapsulated viscumin was released from the microparticles following incubation for 120 h in a phosphate-buffered saline at neutral pH. Importantly, the method of ultrasonic dry supercritical fluid encapsulation failed to alter both the cytotoxic potency and the immunochemical properties of the encapsulated viscumin. Thus, this procedure can be used to generate biodegradable polylactide microparticles with encapsulated bioactive substances.


E. S. Kolotova

Lomonosov Moscow State University

Author for correspondence.
Email: ekaterinakolotova@mail.ru

Russian Federation

S. G. Egorova

Lomonosov Moscow State University

Email: ekaterinakolotova@mail.ru

Russian Federation

A. A. Ramonova

Lomonosov Moscow State University

Email: ekaterinakolotova@mail.ru

Russian Federation

S. E. Bogorodski

Institute of Laser and Information Technologies, Russian Academy of Sciences

Email: ekaterinakolotova@mail.ru

Russian Federation

V. K. Popov

Institute of Laser and Information Technologies, Russian Academy of Sciences

Email: ekaterinakolotova@mail.ru

Russian Federation

I. I. Agapov

Shumakov Federal Research Centre of Transplantology and Artificial Organs

Email: ekaterinakolotova@mail.ru

Russian Federation

M. P. Kirpichnikov

Lomonosov Moscow State University

Email: ekaterinakolotova@mail.ru

Russian Federation

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Copyright (c) 2012 Kolotova E.S., Egorova S.G., Ramonova A.A., Bogorodski S.E., Popov V.K., Agapov I.I., Kirpichnikov M.P.

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