Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells

Cover Page
  • Authors: Shevchuk Z.1, Yurchenko M.Y.2, Darekar S.D.1, Holodnuka-Kholodnyuk I.3, Kashuba V.I.4, Kashuba E.V.1,2
  • Affiliations:
    1. Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
    2. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU
    3. Kirchenstein Institute of Microbiology and Virology, Riga Stradins University
    4. Institute of Molecular Biology and Genetics of NASU
  • Issue: Vol 5, No 1 (2013)
  • Pages: 85-89
  • Section: Research Articles
  • URL: http://actanaturae.ru/2075-8251/article/view/10607
  • DOI: https://doi.org/10.32607/20758251-2013-5-1-85-89
  • Cite item

Abstract


Human mitochondrial ribosomal protein MRPS18-2 (S18-2) is encoded by a cellular gene that is located on the human chromosome 6p21.3. We discovered that overexpression of the S18-2 protein led to immortalization and de-differentiation of primary rat embryonic fibroblasts. Cells showed anchorage-independent growth pattern. Moreover, pathways characteristic for rapidly proliferating cells were upregulated then. It is possible that the S18-2 overexpression induced disturbance in cell cycle regulation. We found that overexpression of S18-2 protein in human cancer cell lines led to an appearance of multinucleated cells in the selected clones.


Z. Shevchuk

Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet

Email: Elena.Kashuba@ki.se

Sweden

M. Y. Yurchenko

Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU

Email: Elena.Kashuba@ki.se

Ukraine

S. D. Darekar

Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet

Email: Elena.Kashuba@ki.se

Sweden

I. Holodnuka-Kholodnyuk

Kirchenstein Institute of Microbiology and Virology, Riga Stradins University

Email: Elena.Kashuba@ki.se

Latvia

V. I. Kashuba

Institute of Molecular Biology and Genetics of NASU

Email: Elena.Kashuba@ki.se

Ukraine

E. V. Kashuba

Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet; Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU

Author for correspondence.
Email: Elena.Kashuba@ki.se

Sweden

  1. Zhang Q.H., Ye M., Wu X.Y., Ren S.X., Zhao M., Zhao C.J., Fu G., Shen Y., Fan H.Y., Lu G. // Genome Research. 2000. V. 10. № 10. Р. 1546-1560.
  2. Suzuki T., Terasaki M., Takemoto-Hori C., Hanada T., Ueda T., Wada A., Watanabe K. // J. Biol. Chem. 2001. V. 276. № 35. Р. 33181-33195.
  3. Cavdar Koc E., Burkhart W., Blackburn K., Moseley A., Spremulli L.L. // J. Biol. Chem. 2001. V. 276. № 22. Р. 19363-19374.
  4. Kashuba E., Pavan Yenamandra S., Deoram Darekar S., Yurchenko M., Kashuba V., Klein G., Szekely L. // Proceedings of the National Academy of Sciences of the United States of America. 2009. V. 106. № 47. Р. 19866-19871.
  5. Yenamandra S.P., Darekar S.D., Kashuba V., Matskova L., Klein G., Kashuba E. // Cell Death Dis. 2012. V. 3. e357.
  6. Snopok B., Yurchenko M., Szekely L., Klein G., Kashuba E. // Anal. and Bioanal. Chem. 2006. V. 386. № 7-8. Р. 2063-2073.
  7. Kashuba E., Yurchenko M., Yenamandra S.P., Snopok B., Isaguliants M., Szekely L., Klein G. // Proceedings of the National Academy of Sciences of the United States of America. 2008. V. 105. № 14. Р. 5489-5494.
  8. Barbera A.J., Chodaparambil J.V., Kelley-Clarke B., Luger K., Kaye K.M. // Cell Cycle. 2006. V. 5. № 10. P. 1048-1052.
  9. Classon M., Harlow E. // Nat. Rev. Cancer. 2002. V. 2. № 12. P. 910-917.
  10. Lussi Y.C., Shumaker D.K., Shimi T., Fahrenkrog B. // Nucleus. 2010. V. 1. № 1. Р. 71-84.
  11. Gupta A., Inaba S., Wong O. K., Fang G., Liu J. // Oncogene. 2003. V. 22. № 48. Р. 7593-7599.
  12. O'Brien T.W. // IUBMB Life. 2003. V. 55. № 9. Р. 505-513.
  13. Saini N., Balhara J., Adlakha Y.K., Singh N. // Int. J. Integ. Biol. 2009. V. 5. № 5. Р. 49-57.
  14. Khanna N., Sen S., Sharma H., Singh N. // Biochem. Biophys. Res. Commun. 2003. V. 30. № 1. Р. 26-35.
  15. Kim M.J., Yoo Y.A., Kim H. J., Kang S., Kim Y.G., Km J.S., Yoo Y.D. // Biochem. Biophys. Res. Commun. 2005. V. 338. № 2. Р. 1179-1184.
  16. Yoo Y.A., Kim M.J., Park J.K., Chung Y.M., Lee J.H., Chi S.G., Kim J.S., Yoo Y.D. // Mol. Cell. Biol. 2005. V. 25. № 15. Р. 6603-6616.

Views

Abstract - 220

PDF (English) - 28

Cited-By


PlumX

Dimensions

Refbacks

  • There are currently no refbacks.

Copyright (c) 2013 Shevchuk Z., Yurchenko M.Y., Darekar S.D., Holodnuka-Kholodnyuk I., Kashuba V.I., Kashuba E.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies