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Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells
- Authors: Shevchuk Z.1, Yurchenko M.Y.2, Darekar S.D.1, Holodnuka-Kholodnyuk I.3, Kashuba V.I.4, Kashuba E.V.1,2
- Affiliations:
- Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
- Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU
- Kirchenstein Institute of Microbiology and Virology, Riga Stradins University
- Institute of Molecular Biology and Genetics of NASU
- Issue: Vol 5, No 1 (2013)
- Pages: 85-89
- Section: Research Articles
- URL: http://actanaturae.ru/2075-8251/article/view/10607
- DOI: https://doi.org/10.32607/20758251-2013-5-1-85-89
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Abstract
Human mitochondrial ribosomal protein MRPS18-2 (S18-2) is encoded by a cellular gene that is located on the human chromosome 6p21.3. We discovered that overexpression of the S18-2 protein led to immortalization and de-differentiation of primary rat embryonic fibroblasts. Cells showed anchorage-independent growth pattern. Moreover, pathways characteristic for rapidly proliferating cells were upregulated then. It is possible that the S18-2 overexpression induced disturbance in cell cycle regulation. We found that overexpression of S18-2 protein in human cancer cell lines led to an appearance of multinucleated cells in the selected clones.
Z. Shevchuk
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
Email: Elena.Kashuba@ki.se
Sweden
M. Y. Yurchenko
Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU
Email: Elena.Kashuba@ki.se
Ukraine
S. D. Darekar
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
Email: Elena.Kashuba@ki.se
Sweden
I. Holodnuka-Kholodnyuk
Kirchenstein Institute of Microbiology and Virology, Riga Stradins University
Email: Elena.Kashuba@ki.se
Latvia
V. I. Kashuba
Institute of Molecular Biology and Genetics of NASU
Email: Elena.Kashuba@ki.se
Ukraine
E. V. Kashuba
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet; Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NASU
Author for correspondence.
Email: Elena.Kashuba@ki.se
Sweden
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