Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase

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Abstract


We unveil experimental evidence that put into question the widely held notion concerning the impact of nanoparticles on the bioelectrocatalytic parameters of enzymatic electrodes. Comparative studies of the bioelectrocatalytic properties of fungal bilirubin oxidase from Myrothecium verrucaria adsorbed on gold electrodes, modified with gold nanoparticles of different diameters, clearly indicate that neither the direct electron transfer rate (standard heterogeneous electron transfer rate constants were calculated to be 31±9 s -1) nor the biocatalytic activity of the adsorbed enzyme (bioelectrocatalytic constants were calculated to be 34±11 s -1) depends on the size of the nanoparticles, which had diameters close to or larger than those of the enzyme molecules.


D. V. Pankratov

A.N. Bach Institute of Biochemistry of Russian Academy of Sciences; National Research Center “Kurchatov Institute”

Author for correspondence.
Email: shleev@inbi.ras.ru

Russian Federation

Y. S. Zeifman

National Research Center “Kurchatov Institute”

Email: shleev@inbi.ras.ru

Russian Federation

А. V. Dudareva

I.G. Petrovsky Bryansk State University

Email: shleev@inbi.ras.ru

Russian Federation

G. K. Pankratova

A.N. Bach Institute of Biochemistry of Russian Academy of Sciences

Email: shleev@inbi.ras.ru

Russian Federation

M. E. Khlupova

A.N. Bach Institute of Biochemistry of Russian Academy of Sciences

Email: shleev@inbi.ras.ru

Russian Federation

Y. M. Parunova

National Research Center “Kurchatov Institute”

Email: shleev@inbi.ras.ru

Russian Federation

D. N. Zajtsev

I.G. Petrovsky Bryansk State University

Email: shleev@inbi.ras.ru

Russian Federation

N. F. Bashirova

I.G. Petrovsky Bryansk State University

Email: shleev@inbi.ras.ru

Russian Federation

V. O. Popov

A.N. Bach Institute of Biochemistry of Russian Academy of Sciences; National Research Center “Kurchatov Institute”

Email: shleev@inbi.ras.ru

Russian Federation

S. V. Shleev

A.N. Bach Institute of Biochemistry of Russian Academy of Sciences; National Research Center “Kurchatov Institute”; I.G. Petrovsky Bryansk State University

Email: shleev@inbi.ras.ru

Russian Federation

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Copyright (c) 2014 Pankratov D.V., Zeifman Y.S., Dudareva А.V., Pankratova G.K., Khlupova M.E., Parunova Y.M., Zajtsev D.N., Bashirova N.F., Popov V.O., Shleev S.V.

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