Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

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Abstract


Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.


M. M. Moisenovich

Biological Faculty, Moscow State University

Author for correspondence.
Email: igor_agapov@mail.ru

Russian Federation

A. Yu. Arkhipova

Biological Faculty, Moscow State University

Email: igor_agapov@mail.ru

Russian Federation

A. A. Orlova

Biological Faculty, Moscow State University

Email: igor_agapov@mail.ru

Russian Federation

M. S. Drutskaya

Biological Faculty, Moscow State University

Email: igor_agapov@mail.ru

Russian Federation

S. V. Volkova

Biological Faculty, Moscow State University

Email: igor_agapov@mail.ru

Russian Federation

S. E. Zacharov

Biological Faculty, Moscow State University

Email: igor_agapov@mail.ru

Russian Federation

I. I. Agapov

Shumakov Institute of Transplantology and Artificial Organs, Federal Agency for High-Tech Medical Services

Email: igor_agapov@mail.ru

Russian Federation

M. P. Kirpichnikov

Biological Faculty, Moscow State University

Email: igor_agapov@mail.ru

Russian Federation

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Copyright (c) 2014 Moisenovich M.M., Arkhipova A.Y., Orlova A.A., Drutskaya M.S., Volkova S.V., Zacharov S.E., Agapov I.I., Kirpichnikov M.P.

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