Analysis of the Placental Tissue Transcriptome of Normal and Preeclampsia Complicated Pregnancies

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  • Authors: Trifonova E.A.1,2, Gabidulina T.V.3, Ershov N.I.4, Serebrova V.N.1, Vorozhishcheva A.Y.5, Stepanov V.A.1,2
  • Affiliations:
    1. Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences
    2. Tomsk State University
    3. Siberian State Medical University, Ministry of Health of the Russian Federation
    4. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
    5. City Clinical Hospital № 1
  • Issue: Vol 6, No 2 (2014)
  • Pages: 71-83
  • Section: Research Articles
  • URL: http://actanaturae.ru/2075-8251/article/view/10553
  • DOI: https://doi.org/10.32607/20758251-2014-6-2-71-83
  • Cite item

Abstract


Preeclampsia is one of the most severe gestational complications which is one of the leading causes of maternal and perinatal morbidity and mortality. A growth in the incidence of severe and combined forms of the pathology has been observed in recent years. According to modern concepts, inadequate cytotrophoblast invasion into the spiral arteries of the uterus and development of the ischemia-reperfusion syndrome in the placental tissue play the leading role in the development of preeclampsia, which is characterized by multipleorgan failure. In this regard, our work was aimed at studying the patterns of placental tissue transcriptome that are specific to females with PE and with physiological pregnancy, as well as identifying the potential promising biomarkers and molecular mechanisms of this pathology. We have identified 63 genes whose expression proved to differ significantly in the placental tissue of females with PE and with physiological pregnancy. A cluster of differentially expressed genes (DEG) whose expression level is increased in patients with preeclampsia includes not only the known candidate genes that have been identified in many other genome-wide studies (e.g., LEP, BHLHB2, SIGLEC6, RDH13, BCL6), but also new genes (ANKRD37, SYDE1, CYBA, ITGB2, etc.), which can be considered as new biological markers of preeclampsia and are of further interest. The results of a functional annotation of DEG show that the development of preeclampsia may be related to a stress response, immune processes, the regulation of cell-cell interactions, intracellular signaling cascades, etc. In addition, the features of the differential gene expression depending on preeclampsia severity were revealed. We have found evidence of the important role of the molecular mechanisms responsible for the failure of immunological tolerance and initiation of the pro-inflammatory cascade in the development of severe preeclampsia. The results obtained elaborate the concept of the pathophysiology of preeclampsia and contain the information necessary to work out measures for targeted therapy of this disease.


E. A. Trifonova

Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences; Tomsk State University

Author for correspondence.
Email: vadim.stepanov@medgenetics.ru

Russian Federation

T. V. Gabidulina

Siberian State Medical University, Ministry of Health of the Russian Federation

Email: vadim.stepanov@medgenetics.ru

Russian Federation

N. I. Ershov

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

Email: vadim.stepanov@medgenetics.ru

Russian Federation

V. N. Serebrova

Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences

Email: vadim.stepanov@medgenetics.ru

Russian Federation

A. Yu. Vorozhishcheva

City Clinical Hospital № 1

Email: vadim.stepanov@medgenetics.ru

Russian Federation

V. A. Stepanov

Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences; Tomsk State University

Email: vadim.stepanov@medgenetics.ru

Russian Federation

  1. Marigorta U.M., Navarro A. // PLoS Genet. 2013, V.9, №6, P.e1003566
  2. Manolio T.A., Collins F.S., Cox N.J., Goldstein D.B., Hindorff L.A., Hunter D.J., McCarthy M.I., Ramos E.M., Cardon L.R., Chakravarti A. // Nature 2009, V.461, №7265, P.747-753
  3. Saccone S.F., Rice J.P., Saccone N.L. // Genet Epidemiol. 2006, V.30, №6, P.459-470
  4. Adzhubei I.A., Schmidt S., Peshkin L., Ramensky V.E., Gerasimova A., Bork P., Kondrashov A.S., Sunyaev S.R. // Nat. Methods. 2010, V.7, №4, P.248-249
  5. Nicolae D.L., Gamazon E., Zhang W., Duan S., Dolan M.E., Cox N.J. // PLoS Genet. 2010, V.6, №4, P.e1000888
  6. Zhong H., Yang X., Kaplan L.M., Molony C., Schadt E.E. // Am. J. Hum. Genet. 2010, V.86, №4, P.581-591
  7. Makarov O.V., Volkova E.V., Jokhadze L.S. // Russian Journal of obstetrician-gynecologist. 2012, №1, P.35-42
  8. Ailamazyan E.K., Mozgovay E.V. // Preeclampsia: theory and practice. M.: MEDpress-Inform, 2008 2008, P.272
  9. George E.M., Granger J.P. // Expert Rev. Obstet. Gynecol. 2010, V.5, №5, P.557-566
  10. Naljayan M.V., Karumanchi S.A. // Adv. Chronic. Kidney Dis. 2013, V.20, №3, P.265-270
  11. // Scientific Center for Obstetrics, Gynecology and Perinatology of Kulakov, the Health Ministry of Russia, Institute for Family Health, The project “Mother and Child”. Hypertension in pregnancy. Preeclampsia. Eclampsia. The clinical protocol. 2012, P.51
  12. Smyth G.K. // Stat. Appl. Genet. Mol. Biol. 2004, V.3, №1, P.1544-6115
  13. Ritchie M.E., Diyagama D., Neilson J., van Laar R., Dobrovic A., Holloway A., Smyth G.K. // BMC Bioinformatics. 2006, V.7, P.e261
  14. Huang da W., Sherman B.T., Tan Q., Kir J., Liu D., Bryant D., Guo Y., Stephens R., Baseler M.W., Lane H.C., Lempicki R.A. // Nucleic Acids Research 2007, V.35, P.169-175
  15. Szklarczyk D., Franceschini A., Kuhn M., Simonovic M., Roth A., Minguez P., Doerks T., Stark M., Muller J., Bork P. // Nucleic Acids Research 2011, V.39, P.561-568
  16. Sitras V., Paulssen R.H., Grønaas H., Leirvik J., Hanssen T.A., Vårtun A., Acharya G. // Placenta. 2009, V.30, №5, P.424-433
  17. Nishizawa H., Pryor-Koishi K., Kato T., Kowa H., Kurahashi H., Udagawa Y. // Placenta. 2007, V.28, №5, P.487-497
  18. Enquobahrie D.A., Meller M., Rice K., Psaty B.M., Siscovick D.S., Williams M.A. // Am. J. Obstet. Gynecol. 2008, V.199, №5, P.e1-11
  19. Founds S.A., Dorman J.S., Conley Y.P. // J. Obstet. Gynecol. Neonatal. Nurs. 2008, V.37, №2, P.146-157
  20. Winn V.D., Gormley M., Paquet A.C., Kjaer-Sorensen K., Kramer A., Rumer K.K., Haimov-Kochman R., Yeh R.F., Overgaard M.T., Varki A. // Endocrinology. 2009, V.150, №1, P.452-462
  21. Lapaire O., Grill S., Lalevee S., Kolla V., Hösli I., Hahn S. // Fetal Diagn. Ther. 2012, V.31, №3, P.147-153
  22. Rogue A., Lambert C., Spire C., Claude N., Guillouzo A. // Drug Metab. Dispos. 2012, V.40, №1, P.151-158
  23. Hulse A.M., Cai J.J. // Genetics. 2013, V.193, №1, P.95-108
  24. Benita Y., Kikuchi H., Smith A.D., Zhang M.Q., Chung D.C., Xavier R.J. // Nucleic Acids Research 2009, V.37, №14, P.4587-4602
  25. Lam K.K., Chiu P.C., Lee C.L., Pang R.T., Leung C.O., Koistinen H., Seppala M., Ho P.C., Yeung W.S. // J. Biol. Chem. 2011, V.286, №43, P.37118-37127
  26. Nichol D., Christian M., Steel J.H., White R., Parker M.G. // J. Biol. Chem. 2006, V.281, №43, P.32140-32147
  27. Huang W., Ghisletti S., Saijo K., Gandhi M., Aouadi M., Tesz G.J., Zhang D.X., Yao J., Czech M.P., Goode B.L. // Nature 2011, V.470, №7334, P.414-418
  28. Gettemans J., van Impe K., Delanote V., Hubert T., Vandekerckhove J., De Corte V. // Traffic. 2005, V.6, №10, P.847-857
  29. Percipalle P. // Nucleus. 2013, V.4, №1, P.43-52
  30. Nishizawa H., Ota S., Suzuki M., Kato T., Sekiya T., Kurahashi H., Udagawa Y. // Reprod. Biol. Endocrinol. 2011, V.2, №9, P.107
  31. Tsai S., Hardison N.E., James A.H., Motsinger-Reif A.A., Bischoff S.R., Thames B.H., Piedrahita J.A. // Placenta. 2011, V.32, №2, P.175-182
  32. Kim J., Zhao K., Jiang P., Lu Z.X., Wang J., Murray J.C., Xing Y. // BMC Genomics. 2012, V.27, №13, P.115
  33. Sood R., Zehnder J.L., Druzin M.L., Brown P.O. // Proc. Natl. Acad. Sci. USA. 2006, V.103, №14, P.5478-5483
  34. Meng T., Chen H., Sun M., Wang H., Zhao G., Wang X. // OMICS. 2012, V.16, №6, P.301-311
  35. Várkonyi T., Nagy B., Füle T., Tarca A.L., Karászi K., Schönléber J., Hupuczi P., Mihalik N., Kovalszky I., Rigó J. Jr. // Placenta. 2011, V.32, SS21, P.9
  36. Lee G.S., Joe Y.S., Kim S.J., Shin J.C. // Arch. Gynecol. Obstet. 2010, V.282, №4, P.363-369
  37. Xiang Y., Cheng Y., Li X., Li Q., Xu J., Zhang J., Liu Y., Xing Q., Wang L., He L., Zhao X. // PLoS One. 2013, V.8, №3, P.e59753
  38. Winn V.D., Gormley M., Fisher S.J. // Pregnancy Hypertens. 2011, V.1, №1, P.100-108
  39. Denver R.J., Bonett R.M., Boorse G.C. // Neuroendocrinology. 2011, V.94, №1, P.21-38
  40. Domali E., Messinis I.E. // J. Matern. Fetal. Neonatal. Med. 2002, V.12, №4, P.222-230
  41. Laivuori H. // Front. Biosci. 2007, V.12, P.2372-2382
  42. Aizawa-Abe M. // J. Clin. Invest. 2000, V.105, P.1243-1252
  43. Fairfax B.P., Vannberg F.O., Radhakrishnan J. // Human Molecular Genetics 2010, V.19, №4, P.720-730
  44. Sugathadasa B.H., Tennekoon K.H., Karunanayake E.H. // Hypertens. Pregnancy. 2010, V.29, P.366-374
  45. Vasku J., Dostalova Z., Kankova K. // J. Obstet. Gynec. 2008, V.34, №5, P.858-864
  46. Jia R.Z., Zhang X., Hu P., Liu X.M., Hua X.D., Wang X., Ding H.J. // Int. J. Mol. Med. 2012, V.30, №1, P.133-141
  47. Hogg K., Blair J.D., von Dadelszen P., Robinson W.P. // Mol. Cell. Endocrinol. 2013, V.367, №1, P.64-73
  48. Rinn J.L., Chang H.Y. // Annu. Rev. Biochem. 2012, V.81, P.145-166
  49. Evdonin A.L., Medvedeva N.D. // Cytology. 2009, V.51, №2, P.130-137
  50. Chernikov V.A., Gorohovets N.V., Savateeva L.V., Severin S.E. // Biomedical Chemistry. 2012, V.58, №6, P.651-661
  51. Abdulsid A., Hanretty K., Lyall F. // PLoS One. 2013, V.8, №1, P.e54540
  52. Cudaback E., Li X., Yang Y., Yoo T., Montine K.S., Craft S., Montine T.J., Keene C.D. // J. Neuroinflammation. 2012, V.10, №9, P.192
  53. Cekmen M.B., Erbagci A.B., Balat A., Duman C., Maral H., Ergen K., Ozden M., Balat O., Kuskay S. // Clin. Biochem. 2003, V.36, №7, P.575-578
  54. Bayhan G., Koçyigit Y., Atamer A., Atamer Y., Akkus Z. // Gynecol. Endocrinol. 2005, V.21, №1, P.1-6
  55. Koçyigit Y., Atamer Y., Atamer A., Tuzcu A., Akkus Z. // Gynecol. Endocrinol. 2004, V.19, №5, P.267-273
  56. Catarino C., Rebelo I., Belo L., Rocha-Pereira P., Rocha S., Castro E.B., Patrício B., Quintanilha A., Santos-Silva A. // Acta Obstet. Gynecol. Scand. 2008, V.87, №6, P.628-634
  57. Ahmadi R., Rahimi Z., Vaisi-Raygani A., Kiani A., Jalilian N., Rahimi Z. // Hypertens. Pregnancy. 2012, V.31, №4, P.405-418
  58. Drigalenko E., Poduslo S., Elston R. // Neurology. 1998, V.51, P.131-135
  59. Ken-Dror G., Talmud P.J., Humphries S.E., Drenos F. // Mol. Med. 2010, V.16, №9, P.389-399
  60. Lucatelli J.F., Barros A.C., Silva V.K., Machado Fda S., Constantin P.C., Dias A.A., Hutz M.H., de Andrade F.M. // Neurochem. Res. 2011, V.36, №8, P.1533-1539
  61. Laresgoiti-Servitje E. // J. Leukoc. Biol. 2013, V.94, №2, P.247-257
  62. Padmini E., Venkatraman U., Srinivasan L. // Toxicol. Mech. Methods. 2012, V.22, №5, P.367-374
  63. Gharesi-Fard B., Zolghadri J., Kamali-Sarvestani E. // Placenta. 2010, V.31, №2, P.121-125

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Copyright (c) 2014 Trifonova E.A., Gabidulina T.V., Ershov N.I., Serebrova V.N., Vorozhishcheva A.Y., Stepanov V.A.

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