Email this article High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis
- Authors: Kapustin D.V.1, Prostyakova A.I.1, Alexeev Y.I.2,3, Varlamov D.A.2,3, Zubov V.P.1, Zavriev S.K.1
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Affiliations:
- M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- ZAO SINTOL
- Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences
- Issue: Vol 6, No 2 (2014)
- Pages: 48-52
- Section: Research Articles
- URL: https://actanaturae.ru/2075-8251/article/view/10546
- DOI: https://doi.org/10.32607/20758251-2014-6-2-48-52
- ID: 10546
Cite item
Abstract
The efficiency of one-step and multi-step protocols of DNA isolation from lysed sputum samples containing the Mycobacterium tuberculosis complex has been compared. DNA was isolated using spin-cartridges containing a special silica-based sorbent modified with fluoroplast and polyaniline, or using an automated isolation system. One-step isolation using the obtained sorbent has been shown to ensure a significantly lower DNA loss and higher sensitivity in the PCR detection of Mycobacterium tuberculosis as compared to a system based on sorption and desorption of nucleic acids during the isolation.
About the authors
D. V. Kapustin
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: kapustin@ibch.ru
Russian Federation
A. I. Prostyakova
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: kapustin@ibch.ru
Russian Federation
Ya. I. Alexeev
ZAO SINTOL; Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences
Email: kapustin@ibch.ru
Russian Federation
D. A. Varlamov
ZAO SINTOL; Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences
Email: kapustin@ibch.ru
Russian Federation
V. P. Zubov
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: kapustin@ibch.ru
Russian Federation
S. K. Zavriev
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: kapustin@ibch.ru
Russian Federation
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