High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis

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  • Authors: Kapustin D.V.1, Prostyakova A.I.1, Alexeev Y.I.2,3, Varlamov D.A.2,3, Zubov V.P.1, Zavriev S.K.1
  • Affiliations:
    1. M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
    2. ZAO SINTOL
    3. Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences
  • Issue: Vol 6, No 2 (2014)
  • Pages: 48-52
  • Section: Research Articles
  • URL: http://actanaturae.ru/2075-8251/article/view/10546
  • DOI: https://doi.org/10.32607/20758251-2014-6-2-48-52
  • Cite item

Abstract


The efficiency of one-step and multi-step protocols of DNA isolation from lysed sputum samples containing the Mycobacterium tuberculosis complex has been compared. DNA was isolated using spin-cartridges containing a special silica-based sorbent modified with fluoroplast and polyaniline, or using an automated isolation system. One-step isolation using the obtained sorbent has been shown to ensure a significantly lower DNA loss and higher sensitivity in the PCR detection of Mycobacterium tuberculosis as compared to a system based on sorption and desorption of nucleic acids during the isolation.


D. V. Kapustin

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Author for correspondence.
Email: kapustin@ibch.ru

Russian Federation

A. I. Prostyakova

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: kapustin@ibch.ru

Russian Federation

Ya. I. Alexeev

ZAO SINTOL; Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences

Email: kapustin@ibch.ru

Russian Federation

D. A. Varlamov

ZAO SINTOL; Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences

Email: kapustin@ibch.ru

Russian Federation

V. P. Zubov

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: kapustin@ibch.ru

Russian Federation

S. K. Zavriev

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: kapustin@ibch.ru

Russian Federation

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Copyright (c) 2014 Kapustin D.V., Prostyakova A.I., Alexeev Y.I., Varlamov D.A., Zubov V.P., Zavriev S.K.

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