Structural Features of the Telomerase RNA Gene in the Naked Mole Rat Heterocephalus glaber

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Abstract


Telomere length, an important feature of life span control, is dependent on the activity of telomerase (a key enzyme of the telomere-length-maintaining system). Telomerase RNA is a component of telomerase and, thus, is crucial for its activity. The structures of telomerase RNA genes and their promoter regions were compared for the long-living naked mole rat and different organisms. Two rare polymorphisms in Heterocephalus glaber telomerase RNA (hgTER) were identified: A→G in the first loop of pseudoknot P2b-p3 (an equivalent of 111nt in hTR) and G→A in the scaRNA domain CR7-p8b (an equivalent of 421nt in hTR). Analysis of TER promoter regions allowed us to identify two new transcription factor binding sites. The first one is the ETS family site, which was found to be a conserved element for all the analyzed TER promoters. The second site is unique for the promoter region of TER of the naked mole rat and is a binding site for the SOX17 transcription factor. The absence of one Sp1 site in the TER promoter region of the naked small rat is an additional specific feature of the promoter area of hgTER. Such variation in the hgTER transcription regulation region and hgTER itself could provide increased telomerase activity in stem cells and an extended lifespan to H. glaber.


S. A. Evfratov

Faculty of Chemistry, Moscow State University

Author for correspondence.
Email: evfratov@gmail.com

Russian Federation

E. M. Smekalova

Faculty of Chemistry, Moscow State University

Email: evfratov@gmail.com

Russian Federation

A. V. Golovin

Faculty of Bioengineering and Bioinformatics, Moscow State University; Belozersky Institute of Physicochemical Biology, Moscow State University

Email: evfratov@gmail.com

Russian Federation

N. A. Logvina

Faculty of Chemistry, Moscow State University

Email: evfratov@gmail.com

Russian Federation

M. I. Zvereva

Faculty of Chemistry, Moscow State University; Belozersky Institute of Physicochemical Biology, Moscow State University

Email: evfratov@gmail.com

Russian Federation

O. A. Dontsova

Faculty of Chemistry, Moscow State University; Belozersky Institute of Physicochemical Biology, Moscow State University

Email: evfratov@gmail.com

Russian Federation

  1. Rando T.A. // Nature 2006, V.441, P.1080-1086
  2. Van Zant G., Liang Y. // Exp. Hematol. 2003, V.31(8), P.659-672
  3. Rossi D.J., Bryder D., Weissman I.L. // Exp. Gerontol. 2007, V.42, P.385-390
  4. Tümpel S., Rudolph K.L. // Ann. N.Y. Acad. Sci. 2012, V.1266, P.28-39
  5. Blasco M.A. // Nat. Chem. Biol. 2007, V.3, P.640-649
  6. Nicholls C., Li H., Wang J.Q., Liu J.P. // Protein Cell. 2011, V.2, P.726-738
  7. Jaskelioff M., Muller F.L., Paik J.H., Thomas E., Jiang S., Adams A.C., Sahin E., Kost-Alimova M., Protopopov A., Cadiñanos J. // Nature 2011, V.469, P.102-106
  8. Bernardes de Jesus B., Vera E., Schneeberger K., Tejera A.M., Ayuso E., Bosch F., Blasco M.A. // EMBO Mol. Med. 2012, V.4, P.691-704
  9. Collins K. // Curr. Opin. Chem. Biol. 2011, V.15, P.643-648
  10. Kelleher C., Teixeira M.T., Forstemann K., Lingner J. // Trends Biochem. Sci. 2002, V.27, P.572-579
  11. Chiodi I., Mondello C. // Front. Oncol. 2012, V.2, P.133
  12. Hiyama E., Hiyama K., Yokoyama T., Shay W.J. // Neoplasia. 2001, V.3, P.17-26
  13. Smekalova E.M., Shubernetskaya O.S., Zvereva M.I., Gromenko E.V., Rubtsova M.P., Dontsova O.A. // Biochemistry (Mosc.) 2012, V.77, P.1120-1128
  14. Chiang Y.J., Hemann M.T., Hathcock K.S., Tessarollo L., Feigenbaum L., Hahn W.C., Hodes R.J. // Mol. Cell. Biol. 2004, V.24, P.7024-7031
  15. Garrels W., Kues W.B., Herrmann D., Holler S., Baulain U., Niemann H. // Biol. Reprod 2012, V.87, P.95
  16. Kim E.B., Fang X., Fushan A.A., Huang Z., Lobanov A.V., Han L., Marino S.M., Sun X., Turanov A.A., Yang P. // Nature 2011, V.479, P.223-227
  17. Yu C., Li Y., Holmes A., Szafranski K., Faulkes C.G., Coen C.W., Buffenstein R., Platzer M., de Magalhães J.P., Church G.M. // PLoS One. 2011, V.6, P.e26729
  18. Gorbunova V., Bozzella M.J., Seluanov A. // Age (Dordr). 2008, V.30, P.111-119
  19. Liang S., Mele J., Wu Y., Buffenstein R. // Aging Cell. 2010, V.9, P.626-635
  20. Sandelin A., Alkema W., Engström P., Wasserman W.W., Lenhard B. // Nucleic Acids Research 2004, V.32, P.D91-D94
  21. Sandelin A., Wasserman W.W., Lenhard B. // Nucleic Acids Research 2004, V.32, P.249-252
  22. Wasserman W.W., Sandelin A. // Nat. Rev. Genet. 2004, V.5, P.276-387
  23. Chen J.L., Blasco M.A., Greider C.W. // Cell. 2000, V.100, P.503-514
  24. Zhao J.Q., Hoare S.F., McFarlane R., Muir S., Parkinson E.K., Black D.M., Keith W.N. // Oncogene. 1998, V.16, P.1345-1350
  25. Makkonen H., Jääskeläinen T., Pitkänen-Arsiola T., Rytinki M., Waltering K.K., Mättö M., Visakorpi T., Palvimo J.J. // Oncogene. 2008, V.27, P.4865-4876
  26. Shore P., Sharrocks A.D. // Nucleic Acids Research 1995, V.23, P.4698-4706
  27. Mo Y., Vaessen B., Johnston K., Marmorstein R. // Molecular Cell 1998, V.2, P.201-212
  28. Wei G.H., Badis G., Berger M.F., Kivioja T., Palin K., Enge M., Bonke M., Jolma A., Varjosalo M., Gehrke A.R. // EMBO J. 2010, V.29, P.2147-2160
  29. Sharrocks A.D., Brown A.L., Ling Y., Yates P.R. // Int. J. Biochem. Cell. Biol 1998, V.29, P.1371-1387
  30. Stros M., Launholt D., Grasser K.D. // Cell. Mol. Life Sci. 2007, V.64, P.2590-2606
  31. Denny P., Swift S., Connor F., Ashworth A. // EMBO J. 1992, V.11, P.3705-3712
  32. Laudet V., Stehelin D., Clevers H. // Nucleic Acids Research 1993, V.21, P.2493-2501
  33. Dunn T.L., Mynett-Johnson L., Wright E.M., Hosking B.M. // Gene. 1995, V.161, P.223-225
  34. Kanai Y., Kanai-Azuma M., Noce T., Saido T.C. // J. Cell. Biol. 1996, V.133, P.667-681
  35. Mertin S., McDowall S.G., Harley V.R. // Nucleic Acids Research 1999, V.27, P.1359-1364
  36. Zhao J.Q., Glasspool R.M., Hoare S.F., Bilsland A., Szatmari I., Keith W.N. // Neoplasia. 2000, V.2, P.531-539
  37. Zhao J., Bilsland A., Hoare S.F., Keith W.N. // FEBS Lett. 2003, V.536, P.111-119
  38. Li L., Davie J.R. // Ann. Anat. 2010, V.192, P.275-283
  39. Séguin C.A., Draper J.S., Nagy A., Rossant J. // J. Cell. Stem Cell. 2008, V.3, P.182-195
  40. Patterson E.S., Addis R.C., Shamblott M.J., Gearhart J.D. // Physiol. Genomics. 2008, V.34, P.277-284
  41. Kim I., Saunders T.L., Morrison S.J. // Cell. 2007, V.130, P.470-483
  42. He S., Kim I., Lim M.S., Morrison S.J. // Genes Dev. 2011, V.25, P.1613-1627
  43. Seluanov A., Chen Z., Hine C., Sasahara T.H. // Aging Cell. 2007, V.6, P.45-52
  44. Vulliamy T., Marrone A., Dokal I., Mason P.J. // Lancet. 2002, V.359, P.2168-2170
  45. Chen J.L., Greider C.W. // Proc. Natl. Acad. Sci. USA. 2005, V.102, P.8080-8085
  46. Vulliamy T., Marrone A., Goldman F., Dearlove A., Bessler M., Mason P.J., Dokal I. // Nature 2001, V.413, P.432-435
  47. Leontis N.B., Westhof E. // RN A. 1998, V.4, P.1134-1153
  48. de Magalhães J.P., Costa J. // J. Evol. Biol. 2009, V.22, P.1770-1774
  49. Theimer C.A., Jády B.E., Chim N., Richard P., Breece K.E., Kiss T., Feigon J. // J. Mol. Cell. 2007, V.27, P.869-881
  50. Jády B.E., Bertrand E., Kiss T. // J. Cell Biol. 2004, V.164, P.647-652
  51. Tycowski K.T., Shu M.D., Kukoyi A., Steitz J.A. // Molecular Cell 2009, V.34, P.47-57
  52. Venteicher A.S., Abreu E.B., Meng Z., McCann K.E., Terns R.M., Veenstra T.D., Terns M.P. // Artandi SE Sci. 2009, V.323, P.644-648

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Copyright (c) 2014 Evfratov S.A., Smekalova E.M., Golovin A.V., Logvina N.A., Zvereva M.I., Dontsova O.A.

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