Specific Visualization of Tumor Cells Using Upconversion Nanophosphors

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  • Authors: Grebenik E.A.1, Generalova A.N.1, Nechaev A.V.2, Khaydukov E.V.3, Mironova K.E.1, Stremovskiy O.A.1, Lebedenko E.N.1, Zvyagin A.V.1,4,5, Deyev S.M.1,5
  • Affiliations:
    1. Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
    2. Lomonosov Moscow State University
    3. Institute of Laser and Information Technologies, Russian Academy of Sciences
    4. Macquarie University
    5. Lobachevsky Nizhniy Novgorod State University
  • Issue: Vol 6, No 4 (2014)
  • Pages: 48-53
  • Section: Research Articles
  • URL: http://actanaturae.ru/2075-8251/article/view/10524
  • DOI: https://doi.org/10.32607/20758251-2014-6-4-48-53
  • Cite item

Abstract


The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the “transparency window” of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase : barstar (Bn : Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn : UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.


E. A. Grebenik

Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Author for correspondence.
Email: katya.ivukina@rambler.ru

Russian Federation

A. N. Generalova

Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: katya.ivukina@rambler.ru

Russian Federation

A. V. Nechaev

Lomonosov Moscow State University

Email: katya.ivukina@rambler.ru

Russian Federation

E. V. Khaydukov

Institute of Laser and Information Technologies, Russian Academy of Sciences

Email: katya.ivukina@rambler.ru

Russian Federation

K. E. Mironova

Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: katya.ivukina@rambler.ru

Russian Federation

O. A. Stremovskiy

Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: katya.ivukina@rambler.ru

Russian Federation

E. N. Lebedenko

Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: katya.ivukina@rambler.ru

Russian Federation

A. V. Zvyagin

Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Macquarie University; Lobachevsky Nizhniy Novgorod State University

Email: katya.ivukina@rambler.ru

Russian Federation

S. M. Deyev

Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Lobachevsky Nizhniy Novgorod State University

Email: katya.ivukina@rambler.ru

Russian Federation

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Copyright (c) 2014 Grebenik E.A., Generalova A.N., Nechaev A.V., Khaydukov E.V., Mironova K.E., Stremovskiy O.A., Lebedenko E.N., Zvyagin A.V., Deyev S.M.

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