Selective Protective Potency of Yersinia pestis ΔnlpD Mutants

Cover Page

Abstract


It has recently been shown that the NlpD lipoprotein is essential to Yersinia pestis virulence and that subcutaneous administration of the nlpD mutant could protect mice against bubonic and pneumonic plague better than the EV vaccine strain [PLoS One 2009. V. 4. № 9. e7023]. In this study, similar ΔnlpD mutants were generated on the basis of other Y. pestis parent strains, including strains from the subspecies microtus, which is avirulent to guinea pigs and humans. Comparative testing confirmed that immunization of mice with ΔnlpD mutants induces immunity 105 times more potent than the one induced by the administration of the EV vaccine strain. At the same time, NlpD- bacteria failed to protect guinea pigs in the case of a subcutaneous challenge with Y. pestis, inducing a 106 times less potent protection compared with that conferred by immunization with the EV vaccine strain. The possible causes of the observed phenomena are discussed.


S. V. Dentovskaya

State Research Center for Applied Microbiology

Email: anisimov@obolensk.org

Russian Federation

S. A. Ivanov

State Research Center for Applied Microbiology

Email: anisimov@obolensk.org

Russian Federation

P. Kh. Kopylov

State Research Center for Applied Microbiology

Email: anisimov@obolensk.org

Russian Federation

R. Z. Shaikhutdinova

State Research Center for Applied Microbiology

Email: anisimov@obolensk.org

Russian Federation

М. E. Platonov

State Research Center for Applied Microbiology

Email: anisimov@obolensk.org

Russian Federation

T. I. Kombarova

State Research Center for Applied Microbiology

Email: anisimov@obolensk.org

Russian Federation

T. V. Gapel’chenkova

State Research Center for Applied Microbiology

Email: anisimov@obolensk.org

Russian Federation

S. V. Balakhonov

Irkutsk Antiplague Research Institute of Siberia and Far East

Email: anisimov@obolensk.org

Russian Federation

A. P. Anisimov

State Research Center for Applied Microbiology

Author for correspondence.
Email: anisimov@obolensk.org

Russian Federation

  1. Naumov A.V., Ledvanov M.Yu., Drozdov I.G. // Immunologiya chumy (Plague immunology). Saratov: Russian Research Anti-Plague Institute “Microbe”, 1992. 172 p. 1992, P.172
  2. Dentovskaya S.V., Kopylov P.Kh., Ivanov S.A., Ageev S.A., Anisimov A.P. // Mol. Genet. Microbiol. Virol. 2013, V.28, P.87-98
  3. Feodorova V.A., Corbel M.J. // Expert Rev. Vaccines. 2009, V.8, P.1721-1738
  4. Firstova V.V., Tyurin E.A., Kravchenko T.B., Zyrina E.V., Biketov S.F., Dyatlov I.A. // Adv. Exp. Med. Biol. 2012, V.954, P.173-177
  5. Li B., Du C., Zhou L., Bi Y., Wang X., Wen L., Guo Z., Song Z., Yang R. // Clin. Vaccine Immunol. 2012, V.19, P.228-234
  6. Smiley S.T. // Adv. Exp. Med. Biol. 2007, V.603, P.376-386
  7. Smiley S.T. // Immunol. Rev. 2008, V.225, P.256-271
  8. Sun W., Roland K.L., Curtiss R. 3rd. // J. Infect. Dev. Ctries. 2011, V.5, P.614-627
  9. Anisimov A.P. // Molecular genetic mechanisms of formation and functional significance of Yersinia pestis capsule. Saratov: Russian Research Anti-Plague Institute “Microbe”, Obolensk: State Research Center for Applied Microbiology, 1999
  10. Korobkova E.I. // Zhivaya protivochumnaya vaktsina (Live antiplague vaccine). Moscow: Medgiz, 1956. 1956, P.208
  11. Girard G. // Biol. Med. (Paris). 1963, V.52, P.631-731
  12. Meyer K.F., Smith G., Foster L., Brookman M., Sung M. // J. Infect. Dis. 1974, V.129, S1, P.S85-S120
  13. Perry R.D., Fetherston J.D. // Clin. Microbiol. Rev. 1997, V.10, P.35-66
  14. Tidhar A., Flashner Y., Cohen S., Levi Y., Zauberman A., Gur D., Aftallon M., Elhanany E., Zvi A., Shafferman A., Mamroud E. // PLoS One. 2009, V.4, P.e7023
  15. Braciale V.L., Nash M., Sinha N., Zudina I.V., Motin V.L. // Correlates of Immunity Elicited by Live Yersinia pestis Vaccine. National Institute of Allergy and Infectious Diseases, NIH. Volume 1, Frontiers in Research.Totowa, NJ: Humana Press Inc., 2007 2007, P.473-480
  16. Derbise A., Cerdà Marín A., Ave P., Blisnick T., Huerre M., Carniel E., Demeure C.E. // PLoS Negl Trop Dis. 2012, V.6, P.1528
  17. Rosenzweig J.A., Chopra A.K. // Expert Rev. Vaccines. 2012, V.11, P.659-661
  18. Sha J., Agar S.L., Baze W.B., Olano J.P., Fadl A.A., Erova T.E., Wang S., Foltz S.M., Suarez G., Motin V.L. // Infect Immun. 2008, V.76, P.1390-1409
  19. Zhang X., Qi Z., Du Z., Bi Y., Zhang Q., Tan Y., Yang H., Xin Y., Yang R., Wang X. // Vaccine. 2013, V.31, P.2539-2542
  20. Flashner Y., Mamroud E., Tidhar A., Ber R., Aftalion M., Gur D., Lazar S., Zvi A., Bino T., Ariel N. // Infect. Immun. 2004, V.72, P.908-915
  21. Garbom S., Forsberg A., Wolf-Watz H., Kihlberg B.M. // Infect. Immun. 2004, V.72, P.1333-1340
  22. Rappuoli R. // Curr. Opin. Microbiol. 2000, V.3, P.445-450
  23. Sun W., Roland K.L., Branger C.G., Kuang X., Curtiss R. // PLoS One. 2009, V.4, P.e6720
  24. Oyston P.C., Mellado-Sanchez G., Pasetti M.F., Nataro J.P., Titball R.W., Atkins H.S. // Microb. Pathog. 2010, V.48, P.191-195
  25. Padmalayam I., Kelly T., Baumstark B., Massung R. // Infect. Immun. 2000, V.68, P.4972-4979
  26. Sha J., Kirtley M.L., van Lier C.J., Wang S., Erova T.E., Kozlova E.V., Cao A., Cong Y., Fitts E.C., Rosenzweig J.A., Chopra A.K. // Infect Immun. 2013, V.81, P.815-828
  27. Woodcock D., Crowther P., Doherty J., Jefferson S., De-Cruz E., Noyer-Weidner M., Smith S., Michael M., Graham M. // Nucleic Acids Res. 1989, V.17, P.3469-3478
  28. Simon R., Priefer U., Pulher A. // Biotechnology. 1983, V.1, P.784-791
  29. Platonov M.E., Evseeva V.V., Dentovskaya S.V., Anisimov A.P. // Mol. Genet. Microbiol. Virol. 2013, V.28, P.41-45
  30. Datsenko K., Wanner B. // Proc. Natl. Acad. Sci. USA. 2000, V.97, P.6641-6645
  31. Donnenberg M.S., Kaper J.B. // Infect. Immun. 1991, V.59, P.4310-4317
  32. Filippov A.A., Solodovnikov N.S., Kookleva L.M., Protsenko O.A. // FEMS Microbiol Lett. 1990, V.55, P.45-48
  33. Filippov A.A., Sergueev K.V., He Y., Huang X.Z., Gnade B.T., Mueller A.J., Fernandez-Prada C.M., Nikolich M.P. // PLoS One. 2011, V.6, P. e25486
  34. Bahmanyar M., Cavanaugh D.C. // Plague manual. Geneva: WHO, 1976. 1976, P.78
  35. Anisimova T.I., Sayapina L.V., Sergeeva G.M., Isupov I.V., Beloborodov R.A., Samoilova L.V., Anisimov A.P., Ledvanov M.Y., Shvedun G.P., Zadumina S.Y. // Main requirements for vaccine strains of the plague pathogen // Methodological Guidelines MU 3.3.1.1113-02 (approved by the Main State Health Officer of Russian Federation on 02.03.2002). Moscow, 2002. 2002, P.69
  36. Qiu Y., Liu Y., Qi Z., Wong W., Kou Z., Zhang Q., Liu G., Yang X., Xin Y., Li C., Scand. J. // Immunol. 2010, V.72, P.425-433
  37. Finney D.J. // Statistical method in biological assay, 3rd Ed. London: Charles Griffin, 1978 1978, P.508
  38. Anisimov A.P., Lindler L.E., Pier G.B. // Intraspecific diversity of Yersinia pestis Clin. Microbiol. Rev. 2004, V.17, P.434-464
  39. Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H. // DNA Res. 2004, V.11, P.179-197
  40. Zhou D., Tong Z., Song Y., Han Y., Pei D., Pang X., Zhai J., Li M., Cui B., Qi Z. J. // Bacteriol. 2004, V.186, P.5147-5152
  41. Kopylov P.Kh., Bakhteeva I.V., Anisimov A.P., Dentovskaja S.V., Ivanov S.A., Kiseleva N.V., Levchuk V.P., Panfertsev E.A., Platonov M.E., Svetoch T.E. // Russian patent RUS 2439155 C1, C12N15/10, C07H21/00, C12N15/70, C12N1/21, C12P21/00, C12R1/19, 2010
  42. Bashaw J., Norris S., Weeks S., Trevino S., Adamovicz J.J., Welkos S. // Clin. Vaccine Immunol. 2007, V.14, P.605-616
  43. Byvalov A.A., Pautov V.N., Chicherin Iu.V., Lebedinski V.A., Evstigneev V.I. // Zh. Mikrobiol. Epidemiol. Immunobiol. 1984, №4, P.74-76
  44. Burrows T.W. // Nature 1957, V.179, P.1246-1247
  45. Hallett A.F., Issacson M., Meyer K.F. // Infect. Immun. 1973, V.8, P.876-881
  46. Jones S.M., Griffin K.F., Hodgson I., Williamson E.D. // Vaccine.2003. V.21, P.3912-3918
  47. Von Metz E., Eisler D.M., Hottle G.A. // Appl. Microbiol. 1971, V.22, P.84-88
  48. Welkos S., Pitt M.L.M. I.O., Martinez M., Friedlander A., Vogel P., Tammariello R. // Vaccine. 2002, V.20, P.2206-2214
  49. Miranda K.L., Poester F.P., Minharro S., Dorneles E.M., Stynen A.P., Lage A.P. // Vaccine. 2013, V.31, P.3014-3018
  50. Brubaker R.R. // Curr. Top. Microbiol. Immunol. 1972, V.57, P.111-158
  51. Meyer K.F. // J. Immunol. 1950, V.64, P.139-163
  52. Schütze H. // Br. J. Exp. Pathol. 1939, V.19, P.293-298

Views

Abstract - 132

PDF (English) - 69

PDF (Russian) - 52

Cited-By


PlumX

Dimensions

Refbacks

  • There are currently no refbacks.

Copyright (c) 2015 Dentovskaya S.V., Ivanov S.A., Kopylov P.K., Shaikhutdinova R.Z., Platonov М.E., Kombarova T.I., Gapel’chenkova T.V., Balakhonov S.V., Anisimov A.P.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies