Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients

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  • Authors: Bryzgunova O.E.1, Tamkovich S.N.1,2, Cherepanova A.V.1, Yarmoshchuk S.V.3, Permyakova V.I.1, Anykeeva O.Y.3, Laktionov P.P.1,3
  • Affiliations:
    1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
    2. Novosibirsk State University
    3. Meshalkin Novosibirsk State Research Institute of Circulation Pathology
  • Issue: Vol 7, No 2 (2015)
  • Pages: 115-118
  • Section: Research Articles
  • URL: http://actanaturae.ru/2075-8251/article/view/10512
  • DOI: https://doi.org/10.32607/20758251-2015-7-2-115-118
  • Cite item

Abstract


A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface. The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total pool of circulating DNA (cfDNA +csbDNA) should be used for a correct analysis of marker DNA concentrations and data standardization.


O. E. Bryzgunova

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences

Email: s.tamk@niboch.nsc.ru

Russian Federation

S. N. Tamkovich

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University

Author for correspondence.
Email: s.tamk@niboch.nsc.ru

Russian Federation

A. V. Cherepanova

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences

Email: s.tamk@niboch.nsc.ru

Russian Federation

S. V. Yarmoshchuk

Meshalkin Novosibirsk State Research Institute of Circulation Pathology

Email: s.tamk@niboch.nsc.ru

Russian Federation

V. I. Permyakova

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences

Email: s.tamk@niboch.nsc.ru

Russian Federation

O. Yu. Anykeeva

Meshalkin Novosibirsk State Research Institute of Circulation Pathology

Email: s.tamk@niboch.nsc.ru

Russian Federation

P. P. Laktionov

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences; Meshalkin Novosibirsk State Research Institute of Circulation Pathology

Email: s.tamk@niboch.nsc.ru

Russian Federation

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Copyright (c) 2015 Bryzgunova O.E., Tamkovich S.N., Cherepanova A.V., Yarmoshchuk S.V., Permyakova V.I., Anykeeva O.Y., Laktionov P.P.

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