Thio Derivatives of 2(5H)-Furanone As Inhibitors against Bacillus subtilis Biofilms

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Abstract


Gram-positive bacteria cause a wide spectrum of infectious diseases, including nosocomial infections. While in the biofilm, bacteria exhibit increased resistance to antibiotics and the human immune system, causing difficulties in treatment. Thus, the development of biofilm formation inhibitors is a great challenge in pharmacology. The gram-positive bacterium Bacillus subtilis is widely used as a model organism for studying biofilm formation. Here, we report on the effect of new synthesized 2(5Н)-furanones on the biofilm formation by B.subtilis cells. Among 57 compounds tested, sulfur-containing derivatives of 2(5H)-furanone (F12, F15, and F94) repressed biofilm formation at a concentration of 10 μg/ml. Derivatives F12 and F94 were found to inhibit the biosynthesis of GFP from the promoter of the eps operon encoding genes of the biofilm exopolysaccharide synthesis (EPS). Using the differential fluorescence staining of alive/dead cells, we demonstrated an increased bacterial sensitivity to antibiotics (kanamycin and chloramphenicol) in the presence of F12, F15, and F94, with F12 being the most efficient one. The derivative F15 was capable of disrupting an already formed biofilm and thereby increasing the efficiency of antibiotics.


E. Yu. Trizna

Kazan (Volga Region) Federal University

Author for correspondence.
Email: trizna91@mail.ru

Russian Federation

E. N. Khakimullina

Kazan (Volga Region) Federal University

Email: trizna91@mail.ru

Russian Federation

L. Z. Latypova

Kazan (Volga Region) Federal University

Email: trizna91@mail.ru

Russian Federation

A. R. Kurbangalieva

Kazan (Volga Region) Federal University

Email: trizna91@mail.ru

Russian Federation

I. S. Sharafutdinov

Kazan (Volga Region) Federal University

Email: trizna91@mail.ru

Russian Federation

V. G. Evtyugin

Kazan (Volga Region) Federal University

Email: trizna91@mail.ru

Russian Federation

E. V. Babynin

Kazan (Volga Region) Federal University

Email: trizna91@mail.ru

Russian Federation

M. I. Bogachev

St. Petersburg State Electrotechnical University

Email: trizna91@mail.ru

Russian Federation

A. R. Kayumov

Kazan (Volga Region) Federal University

Email: trizna91@mail.ru

Russian Federation

  1. Vlamakis H., Chai I.O., Y. I.O., Beauregard P., Losick R., Kolter R. // Nat. Rev. Microbiol. 2013, №11, P.15-168
  2. Kayumov A.R., Balaban N.P., Mardanova A.M., Kostrov S.V., Sharipova M.R. // Microbiology. 2006, V.75, №5, P.557-563
  3. Schmidt T., Ziganshin A.M., Nikolausz M., Scholwin F., Nelles M., Kleinsteuber S., Pröter J. // Biomass Bioenergy. 2014, V.69, P.241-248
  4. Ma Y., Xu Y., Yestrepsky B.D., Sorenson R.J., Chen M., Larsen S.D., Sun H. // PLoS One. 2012, P.1-10
  5. Lonn-Stensrud J., Landin M.A., Benneche T., Petersen F.C., Scheiel A.A. // J. Antimicrobial Chemothera P.309-316
  6. Kayumov A.R., Khakimullina E., Sharafutdinov I., Trizna E., Latypova L., Hoang L., Margulis A., Bogachev M., Kurbangalieva A. // J. Antibiotics. 2014, №143, P.doi:10.1038
  7. Kurbangalieva A.R., Devyatova N.F., Bogdanov A.V., Berdnikov E.A., Mannafov T.G., Krivolapov D.B., Litvinov I.A., Chmutova G.A. // Phosphorus, Sulfur, Silicon, Relat. Elem. 2007, V.182, №3, P.607-630
  8. Latypova L.Z., Saygitbatalova E.Sh., Chulakova D.R., Lodochnikova O.A., Kurbangalieva A.R., Berdnikov E.A., Chmutova G.A. // Rus. J. Org. Chem. 2014, V.50, №4, P.532-545
  9. Khoang T.L., Khaziev R.M., Zaripova A.R., Kurbangalieva A.R., Chmutova G.A. // Book of Abstracts of the III All- Russian Scientific Conference “Progress in Synthesis and Complex Formation”. Moscow, April 21-25, 2014. Moscow: RUDN, 2014, P.205
  10. Kayumov A., Heinrich A., Sharipova M., Iljinskaya O., Forchhammer K. // Microbiology. 2008, V.154, P.2348-2355
  11. Kobayashi K. // Mol. Microbiol. 2008, №69, P.1399-1410
  12. Ames B.N., McCann J. // Ann. N.Y. Acad. Sci. 1976, V.271, P.5-13
  13. Oda Y., Nakamura S., Oki I., Kato T., Shinagawa H. // Mutat Res. 1985, V.147, P.219-229
  14. Sambrook J., Fritsch E.F., Maniatis T. // Molecular cloning. A laboratory manual. N.Y.: Cold Spring Harbor Lab. 1989, P.4-16
  15. Kayumov A., Heinrich A., Sharipova M., Iljinskaya O., Forchhammer K. // Microbiology. 2008, V.154, P.2348-2355
  16. Fedorova K., Kayumov A., Woyda K., Ilinskaja O., Forchhammer K. // FEBS Lett. 2013, V.587, P.1293-1298
  17. Prichard M.N., Jr. Shipman C. // Antiviral Res. 1990, №14, P.181-206

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Copyright (c) 2015 Trizna E.Y., Khakimullina E.N., Latypova L.Z., Kurbangalieva A.R., Sharafutdinov I.S., Evtyugin V.G., Babynin E.V., Bogachev M.I., Kayumov A.R.

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